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. 2018 Jul 2;217(7):2503–2518. doi: 10.1083/jcb.201711037

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© 2018 Biedka et al.

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Figure 8. — AFs bound to the ITS2 foot are present in cytoplasmic pre-60S subunits in the absence of Las1. (A and B) Pre-60S subunits containing (gal) or lacking (glu) Las1 were purified using TAP-tagged Nmd3 as bait. The protein composition of preribosomes was analyzed by SDS-PAGE followed by silver staining (A) and Western blotting (B). (C) The cellular localization of GFP-tagged Nop7 and Cic1 was monitored in live yeast cells expressing (gal) or depleted of (glu) Las1. Bar, 5 µm. (D) The locations of AFs bound on or proximal to ITS2 in the state E particle. The structure shown is PDB ID 6ELZ.