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Validation of import cargo candidates. The analysis of potential import cargo candidates was performed as described in Fig. 4 and included the human proteins Hat1 (O14929), Nampt (P43490), Smug1 (Q53HV7), Hmbs (P08397), and Hdac8 (Q9BY41). Note that the anti-Xpo7 nanobodies shift the here-validated import cargoes to a more cytoplasmic localization. Error bars represent SD. ***, p < 10−6. Bar, 20 µm.
