FIGURE 1.

Voltage dependent potassium (K_V) current properties of NMc neurons. (A) Representative membrane response recorded from an NMc neuron to current injection of 200 pA for 100 ms. (B) Representative membrane response recorded from a mid- to high-frequency (M-HF) NM neuron to current injection of 500 pA for 100 ms. (C) Average population data showing differences in the number of APs as a function of current injection for NMc and mid- to high-frequency (M-HF) NM neurons. Data in (C) were modified from our previous study (see Wang et al., 2017; Figure 10D). (D) Representative K_V current traces (I_K) recorded from an NMc neuron, in response to membrane voltages clamped from –100 to +20 mV (Δ step = 5 mV, duration = 100 ms). The holding voltage is –70 mV. Steady-state (SS) K_V currents were measured at the end of current traces (filled circle). (E) Population data showing the relationship of steady-state (SS) K_V currents to membrane voltages (V_MEMBRANE). Note that data point at –100 mV is not shown for simplicity. (F) Population data showing the K_V current density (ρ_K) and conductance (G_K) at the membrane voltage (V_MEMBRANE) of +20 mV. Numbers in bars represent sample size. (G) Representative K_V current traces (I_K) in control and during drug application. Flx, fluoxetine (100 μM). DTx, dendrotoxin (0.1 μM). Symbols (circle, square, and diamond) at the end of current traces represent time window of measured steady-state (SS) K_V currents. (H) Population data showing the relationship of steady-state (SS) K_V currents to membrane voltages (V_MEMBRANE) in control and during drug application. (I,J) Population data showing the percent reduction in steady-state (SS) K_V currents due to the application of Flx (I) or DTx (J), at membrane voltages (V_MEMBRANE) of +20, –10 and –45 mV. Error bar = standard error.