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. 2018 Jul 2;8:9943. doi: 10.1038/s41598-018-28158-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differentially expressed genes (DEGs) analysis between G and R stages. (a) Number of upregulated and downregulated unigenes in R stage compared to G stage were identified by three methods Kallisto, DESeq2, and EdgeR. Bars represent the numbers of bilberry unigenes identified as DEGs. Numbers on the top of bars indicate the number of upregulated and downregulated DEGs in R stage compared to G stage, respectively. G and R indicate two bilberry developmental stages which are used for transcriptome libraries. Venn diagrams represent the comparison of up- and downregulated DEGs among Kallisto, DESeq2, EdgeR methods (b) GO enrichment analysis of bilberry DEGs. Bars represent the numbers of up- and downregulated DEGs in R stage compared to G stage which were assigned into 37 over-represented GO terms (p-value < 0.05) of three main categories: BP = Biological process, CC = Cellular component, MF = Molecular function.