Figure 4.

qRT-PCR analysis of TFs during bilberry fruit development. (a) Five development stages of fruit during ripening process. S1–Flower, S2–Small unripe green fruit, S3–Large unripe green fruit (G), S4–Ripening purple fruit (R), S5–Fully ripe blue fruit. G and R indicated two bilberry stages which are used for construction of transcriptome libraries. Relative expression of (b–f) NAC, (g–i) WRKY, (j) LOB = Lateral Organ Boundaries domain, (k) SPATULA, (i–o) MADS. Bars represent the relative expression levels of unigenes in each stage normalized with respect to the internal control GAPDH. Error bars represent standard error of four biological replicates. Asterisks indicate significant differences between early stage (S3) and ripening stages (S4, S5) at level ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 using Student’s t-Test.