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. 2018 Jul 2;8:9943. doi: 10.1038/s41598-018-28158-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qRT-PCR analysis of hormone-related TFs during bilberry fruit development. (a–e) ERF = Ethylene Responsive Transcription Factor, (f) EIL = Ethylene Insensitive like, (g,h) AP2/ERF = APETALA2/ Ethylene Responsive transcription Factor, (i,j) ARF = Auxin Response Factor, (k,l) ABI = Abscisic acid Insensitive. Bars represent the relative expression levels of unigenes in each stage normalized with respect to the internal control GAPDH. Error bars represent standard error of four biological replicates. S1–Flower, S2–Small unripe green fruit, S3–Large unripe green fruit (G), S4–Ripening purple fruit (R), S5–Fully ripe blue fruit. G and R indicated two bilberry stages which are used for construction of transcriptome libraries. Asterisks indicate significant differences between early stage (S3) and ripening stages (S4, S5) at level ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 using Student’s t-Test.