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. 2018 Jul 2;8:9919. doi: 10.1038/s41598-018-28227-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of MEC size in healthy and diseased lacrimal glands. (A) Lacrimal glands from BALB/c mice were stained with an antibody against α-smooth muscle actin (SMA) and imaged by confocal microscopy to visualize whole MECs. (B) ImageJ was used to create masks of MECs in the normal lacrimal gland to generate the representative sizes. The same method was then used to compare MEC size, using SMA staining (green; C,E) in lacrimal glands of diseased and healthy mice. MEC staining was masked using ImageJ (white; C,E) and quantified to determine the average size of the stained fragments to compare NOD and BALB/c (C,D) or MRL/lpr and MRL +/+ (E,F). Star denotes statistically significant difference compared to control; n = 6 for MRL/lpr mice and n = 5 for all other groups; scale bar represents 25 µm (A) and 50 µm (C,E).