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. 2018 Jul 2;8:9919. doi: 10.1038/s41598-018-28227-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Decrease in contraction of MECs from NOD mice. Lobules prepared from the lacrimal glands of NOD and BALB/c mice were treated with oxytocin or PBS (vehicle) to stimulate MECs contraction. (A,C) show the basal acinus size in non-stimulated lacrimal glands from BALB/c and NOD mice respectively, while (B,D) show the acinus size after stimulation with 5 nM oxytocin for these mice; respectively. The acinus size was measured using 3 metrics, as depicted in (E). Acinus size was compared using (F) area, (G) perimeter, and (H) Feret’s diameter. Star indicates statistically significant difference compared to the indicated group; n = 3 per group.