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. 2018 Jun 26;9:675. doi: 10.3389/fphar.2018.00675

FIGURE 3.

FIGURE 3

Influence of α7nAChR antagonist on the anti-arthritic effect of sinomenine (SIN). Rats were intragastrically administered with SIN and intraperitoneally administered with α-bungarotoxin (α-BTX) or nicotine (NIC) from day 14 to day 28. α-BTX was given 30 min before the administration of vehicle or SIN. (A) The paw swelling and arthritis indexes were determined. (B) Photographs of representative hind paws were taken, and histopathological changes of right ankle joints in each group were determined by HE staining (original magnification 200×). (C) The histological scores of each group were calculated. (D) The serum levels of TNF-α, IL-6, and IL-10 were measured by ELISA. (E) The theoretical possibility of SIN binding to α7nAChR was illustrated by molecular docking. Data were expressed as means ± SEM, n = 6–8. F-values were 13.02 for paw swelling and 8.67 for arthritis index in (A). #P < 0.05 and ##P < 0.01 versus Normal; ∗P < 0.05 and ∗∗P < 0.01 versus Model; &P < 0.05 and &&P < 0.01 versus SIN.