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. 2018 Jul 2;8:9944. doi: 10.1038/s41598-018-28239-7

Figure 1.

Figure 1

RNA Sequencing of fluorescence-activated cell sorted (FACS) postnatal day 21 (P21) mouse Purkinje cells (PC) and ventricular myocytes (VM). (A) Expression of PC (n = 7) and VM (n = 10) transcription factors (TFs) at P21. Differential expression (PC vs VM) on the X-axis and Purkinje transcript levels (normalized counts) are shown on the Y-axis. Significantly enriched (Padj < 0.05) and top ten abundant PC-TFs labeled in green while all remaining significantly different TFs were labeled in red. TFs that were not significantly different labeled in black. (B) Top ten significantly enriched and abundant TFs in PCs. Etv1 was the most enriched and one of the most abundant TFs within PCs. (C,D) Etv1-enhanced green fluorescent protein (EGFP) bacterial artificial chromosome (BAC) transgenic mice were used to confirm regional expression of Etv1 in embryonic (C) and adult (D) hearts. (C) Immunohistochemistry on E13.5 Etv1-EGFP hearts showing overlapping expression of Connexin-40 (Cx40) and GFP in trabecular and atrial myocytes. Bottom two rows are high-magnification views of the Bundle of His (red dashed box) and Trabecular/Compact zone myocytes (white dashed box) outlined in the top row (D) Immunohistochemistry on P70 Etv1-EGFP hearts showing overlapping expression of Cx40 and GFP in atrial myocytes (top row), bundle of His (middle row), and PCs (bottom row). Scale bars: 100 μm (top) 50 μm (middle, bottom) (C); 25 μm (D).