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Electron micrographs of autophagosomes in the sec18 mutant. Wild-type (A) and sec18 (B and C) cells were cultured in SD(-N) containing PMSF at 37°C for 2.5 h and fixed by the freeze substitution technique as described in MATERIALS AND METHODS. Higher magnification images of the autophagosomes in sec18 are shown in D and E. Arrows, autophagosome; arrowheads, autophagic body; V, vacuole. Bars, 500 nm (A–C); 100 nm (D and E).
