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API transport was impaired in sec12Δcvt9 cells. (A) Wild-type (wt), Δcvt9, sec12Δcvt9, and sec12 cells grown in YPD at 23°C were cultured at 37°C in YPD or SD(-N) for 3 h, lysed, and analyzed by immunoblotting with anti-API serum. (B) The mutant cells in A were cultured in YPD at 23°C (open bar), transferred to SD(-N) at 23°C (shaded bar) or 34°C (filled bar) for 3 h, lysed, and assessed for ALP activity.
