Figure 8.

Autophagosome formation in sec12 and sec18 cells. (A) Δypt7 sec12 cells were cultured in YPD or SD(-N) at 37°C for 3 h. Fractionation and protease treatment were performed as in Figure 2. (B) Δypt7, sec18, and Δypt7 sec12 cells precultured in YPD at 23°C were cultured in YPD or SD(-N) at 37°C for 3 h. Density gradient centrifugation and immunoblotting were carried out as described in Figure 7. As a control, one-tenth of the total cell lysate and half of the LSP were also analyzed.