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. 2018 Jun 26;9:1396. doi: 10.3389/fmicb.2018.01396

FIGURE 2.

FIGURE 2

(A) Time course of persiter cell retention in E. coli. W3110 cells were incubated in fresh LB containing amp at 37°C up to 28 days. Data on retention of persister cells (log % survival) are presented as means and S.D. (∗t-test: P < 0.05, n = 5–7; ∗∗t-test: P < 0.01, n = 5–7; ∗∗∗t-test: P < 0.005, n = 5–7). Data are shown for cells grown in liquid (light gray line) and colony–biofilm culture (dark gray line). (B) Time course of persiter cell retention in several bacterial species. E. coli BW25113 (a–c) and several bacteria [Salmonella Typhimurium (d), Acinetobacter radioresistens (e), Bacillus subtilis (f), and Staphylococcus epidermidis (g)] were used. Antibiotics [kan (40 μg/mL for Acinetobacter and Staphylococcus), cam (50 μg/mL for Bacillus), and ofl (5 μg/mL for Salmonella)] were chosen in pilot testing. Data on retention of persister cells (log % survival) are presented as means and S.D. (∗t-test: P < 0.05, n = 4; ∗∗t-test: P < 0.01, n = 4; ∗∗∗t-test: P < 0.005, n = 4). Data are shown for cells grown in liquid (light gray line) and colony biofilm culture (dark gray line).