Fig. 3.

Human mo-DCs and mo-Mac both cross-present efficiently. Purified DCs and macrophages from tumor ascites (a) or in vitro culture of monocytes (b, c), or DCs derived in vitro from CD34⁺ precursors (d) were incubated with serial concentrations of MelanA long or short peptide (a, b, d) or MelanA-coated beads (c). After washing, antigen-specific CD8⁺ T cells were added. After 24 h, IFN-γ secretion was assessed as a measure of T cell activation. Background level was subtracted. Mean ± SEM of three (a, d), six (b), or five (c) independent experiments