Fig. 5.

Human mo-DCs and mo-Mac use the vacuolar pathway for cross-presentation. Purified in vitro-generated mo-DCs (a, d), mo-Mac (b, e), or CD34⁺ cell-derived CD1a⁺ DCs (c, f) were incubated with serial concentrations of MelanA long or short peptide, in the absence or presence of lactacystin (a–c) or pan-cathepsin inhibitor (d–f). After washing, antigen-specific CD8⁺ T cells were added. After 24 h, IFN-γ secretion was assessed as a measure of T cell activation. Background level was subtracted. Mean ± SEM of three (a), five (b, d, e), or three (c, f) independent experiments