Table 3.
Kinetic properties of XoxF4-1, XoxF4-2, and XoxF5 enzymes.
| Source |
Methylotenera mobilis JLW8 XoxF4-1 |
No |
Methylotenera mobilis JLW8 XoxF4-2 |
No |
Methylomonas sp. LW13 XoxF5 |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Catalytic properties | Vmax (U/mg) | Km (mM) | Keff# (s-1 mM-1) | Vmax (U/mg) | Km (mM) | Keff (s-1 mM-1) | Vmax (U/mg) | Km (mM) | Keff (s-1 mM-1) | No | ||
| Methanol | 0.65 ± 0.56 | 0.055 ± 0.032 | 27.17 ± 19.53 | 5 | 0.78 ± 0.30 | 0.042 ± 0.018 | 42.19 ± 17.19 | 5 | 5.97 ± 0.94 | 0.039 ± 0.011 | 342.55 ± 98.94 | 5 |
| Ethanol | 0.79 ± 1.26 | 0.059 ± 0.042 | 27.03 ± 28.94 | 3 | 0.94 ± 0.80 | 0.515 ± 1.211 | 5.99 ± 8.15 | 3 | 6.02 ± 3.58 | 0.085 ± 0.044 | 159.15 ± 150.26 | 3 |
| Propanol | 1.00 | 0.061 | 35.36 | 1 | 0.69 | 0.873 | 1.73 | 1 | 6.88 ± 20.65 | 0.133 ± 0.508 | 112.54 ± 99.11 | 2 |
| Butanol | 1.45 | 0.188 | 16.63 | 1 | 0.46 | 0.47 | 2.15 | 1 | 5.59 ± 2.07 | 0.096 ± 0.070 | 133.90 ± 118.38 | 3 |
| Hexanol | 0.62 ± 0.49 | 0.037 ± 0.163 | 34.88 ± 26.24 | 5 | 0.86 ± 0.69 | 0.357 ± 0.204 | 5.54 ± 3.31 | 4 | 6.32 ± 2.07 | 0.04 ± 0.037 | 415.52 ± 187.38 | 5 |
| Formaldehyde | 0.85 ± 0.93 | 0.432 ± 0.163 | 3.97 ± 344 | 4 | 0.95 ± 1.32 | 0.815 ± 0.748 | 2.48 | 3 | 6.26 ± 1.03 | 0464 ± 0.456 | 37.86 ± 19.57 | 5 |
| Subunit | α2 | α2 | α2 | |||||||||
| Molecular mass (kDa) | 129.4 | 131.7 | 127.9 | |||||||||
Values of Vmax, Km, and Keff were determined in one to five independent experiments, as indicated. The data were merged by 95% confidence interval (CI). 95% CI = x ±t∗s/√n, where x = average activity, s = standard deviation, t = TINV (0.05, degrees of freedom), and n = sample number. #Keff = Kcat/Km.