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. 2018 Jun 26;9:1395. doi: 10.3389/fmicb.2018.01395

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Copyright © 2018 Tsitsopoulou, Posso, Vale, Bebb, Johnson and White.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pathonostics Aspergenius® melt-curve analysis for the TR34/L98H and T289A/Y121F mutations, with typical melting temperatures for wild-type (WT) and mutations (M) indicated by the respective lines with initial testing direct from VIPcheck™ plate showing ambiguous results (A), repeat testing from VIPcheck™ plate showing the TR34/L98H mutation (B) and repeat testing from purity plate showing the T289A/Y121F mutation (C). The control is represented by the black line and the isolate is represented by the gray line.