Figure 1.

Structure of the 75.6 kb ICE within the chromosome of P. multocida strain 3358 is depicted by the blue region, with the extent of the two accessory regions indicated in pale red. The size marker dots are spaced at 500 bp. The int1, int2, ICE-rel1, and parB genes in the core regions are shown in green, other essential core genes in yellow, the remaining core genes in black, and antibiotic resistance genes in the accessory regions in red. Genes are annotated according to their known functions or by using the NCBI notation (Supplementary Table S1). All numbered genes are prefixed with pmu3358_ as shown for the first and last genes in this figure. The hybridization sites for the primers used in the multiplex PCR assays are indicated by the arrow heads below the respective genes.