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. 2018 Jun 26;9:1329. doi: 10.3389/fmicb.2018.01329

Figure 4.

Figure 4

Relative numbers of the P. multocida (blue) and M. haemolytica strains (green) containing the ICE core genes screened in the multiplex PCR assay. Twenty-four of the 43 P. multocida isolates gave a positive signal with each of the int1, int2, ICE-rel1, and parB primer combinations; and a similar proportion of M. haemolytica strains (17 of 32) was also shown to contain these four ICE genes. The remainder of the P. multocida strains tested lacked all of the int1, int2, ICE-rel1, and parB genes, but no P. multocida strain was found to harbor a truncated, nonfunctional ICE structure. In contrast, 14 M. haemolytica strains possessed degenerate ICE sequences that would presumably not be capable of promoting intercellular transfer.