Figure S2.

HPLC analysis for purified recombinant HbA and HbF.

Reverse phase HPLC analysis of rHbA (green-280 nm, red-400 nm) or rHbF (blue-280 nm, orange-400 nm) after purification. Heme (iron-protoporphyrin IX) elutes at 13.5 min (off scale for 400 nm). Hb-β elutes at 22.35 min, with both Hb-α and Hb-γ co-eluting at 24.15 min. Other heme-like compounds absorbing at 400 nm are less than 0.3 % of the heme concentration based on the integration of peaks at 400 nm. rHbA and rHbF signals are offset by 10 and 30 milli-AU for clarity.

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