Figure S1.

SDS PAGE electrophoresis for recombinant HbA and HbF.

rHbA or rHbF (both 9uM) were subjected to electrophoresis on a 12% Mini Protean TGX Stain Free Gel (BioRad) at 170 V for 30 min. The gel was exposed to UV light for 2 min before visualising. Lanes 2 and 5 represent rHbA and rHbF after purification using CM-Sepharose column, lanes 3 and 6 represent the proteins in the same order after purification using Q-Sepharose column and lanes 4 and 7 after the HisTrap HP column). M = Hb monomer, D = Hb dimer.

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