Figure 2.

Time-course of the ascorbic acid uptake in reconstituted proteoliposomes. The uptake was started by adding 0.1 mM external [¹⁴C]ascorbic acid at time zero to proteoliposomes reconstituted with HTP eluate from rat liver (A) or potato (B). Proteoliposomes were reconstituted without (◦,■,Δ,▴,∇) or with 10 mM ascorbic acid (•) or 10 mM DHA (□) as internal substrate. Transport was stopped by adding 15 mM PLP after the indicated time intervals. Data are means ± SD from three independent experiments.