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. 2018 Jun 26;12:38. doi: 10.3389/fninf.2018.00038

Figure 7.

Figure 7

Reproducibility example: multiple experimental inputs converging onto a common signaling pathway (MAPK). (A) Block diagram of composite model. Sub-models for the different inputs are indicated in green (epidermal growth factor, EGF), red (BDNF) and blue (Calcium). (B) Response to EGF stimulus is a transient activation of MAPK. Model (dashed green line) closely follows experimental curve (solid green line). (C) Response to EGF in similar preparation but lower dose. Note that the simulated peak response is higher than experiment in (B), but lower in (C), leading to difficulties in model fitting. (D) Response to BDNF stimulus is also a transient, but the time-course of MAPK signaling in this experiment is much longer than it was in panel (B). (E) Response to an LTP-induction stimulus for calcium, consisting of three pulses separated by 600 s. Here the reference is an earlier simulation predicting sustained activation of MAPK. Remarkably, there is a reasonable match to the reference behavior in all three cases, despite the inputs converging onto the MAPK pathway through different signaling pathways, and the results drawn from very different data sources.