Table 2.
The activity data of compounds 19–34 expressed as MIC (MBC or MFC) [µg/ml] and {MBC/MIC or MFC/MIC} against the reference strains of bacteria and fungi
Species | MIC (MBC/MFC) [µg/ml] and {MBC/MIC or MFC/MIC} of the tested compounds | MIC (MBC) [µg/ml] of reference substances | |||||||
---|---|---|---|---|---|---|---|---|---|
CIP/NY* | NIT | CFX | APC | PA | |||||
19 | 20 | 21 | 34 | ||||||
Gram-positive bacteria | |||||||||
Staphylococcus aureus ATCC 25923 | 1000 (> 1000) {nd} |
1000 (> 1000) {nd} |
500 (> 1000) {nd} |
1000 (> 1000) {nd} |
0.488 | 15.62 | 0.49 | nd | 31.25 (62.5) |
Staphylococcus aureus ATCC 6538 | 1000 (> 1000) {nd} |
– | 500 (> 1000) {nd} |
500 (> 1000) {nd} |
0.244 | 15.62 | 0.98 | nd | 62.5 (125) |
Staphylococcus aureus ATCC 43300 | 1000 (> 1000) {nd} |
– | 1000 (> 1000) {nd} |
1000 (> 1000) {nd} |
0.244 | 7.81 | nd | nd | 62.5 (125) |
Staphylococcus epidermidis ATCC 12228 | 1000 (> 1000) {nd} |
– | 1000 (> 1000) {nd} |
1000 (> 1000) {nd} |
0.122 | 3.91 | 0.24 | nd | 15.62 (62.5) |
Micrococcus luteus ATCC 10240 | 500 (> 1000) {nd} |
1000 (> 1000) {nd}) |
500 (> 1000) {nd} |
500 (> 1000) {nd} |
0.976 | 62.5 | 0.98 | nd | 125 (250) |
Bacillus subtilis ATCC 6633 | 500 (> 1000) {nd} |
– |
500
(1000) {2} |
1000 (> 1000) {nd} |
0.031 | 3.91 | 15.62 | nd | 3.91 (7.81) |
Bacillus cereus ATCC 10876 | 1000 (> 1000) {nd} |
1000 (> 1000) {nd} |
500 (> 1000) {nd} |
1000 (> 1000) {nd} |
0.061 | 7.81 | 31.25 | 62.5 | 15.62 (62.5) |
Gram-negative bacteria | |||||||||
Bordetella bronchiseptica ATCC 4617 | – | 1000 (> 1000) {nd} |
1000 (> 1000) {nd} |
– | 0.976 | 125 | nd | nd | 31.25 (62.5) |
Klebsiella pneumoniae ATCC 13883 | – | – | – | – | 0.122 | 15.62 | nd | nd | 15.62 (31.25) |
Proteus mirabilis ATCC 12453 | – | – | – | – | 0.030 | 62.5 | nd | nd | 1.95 (3.91) |
Salmonella typhimurium ATCC 14028 | – | – | – | – | 0.061 | 31.25 | nd | nd | 1.95 (3.91) |
Escherichia coli ATCC 25922 | – | – | – | – | 0.004 | 7.81 | nd | nd | 0.98 (1.95) |
Pseudomonas aeruginosa ATCC 9027 | – | – | – | – | 0.488 | nd | nd | nd | 31.25 (62.5) |
Fungi | |||||||||
Candida albicans ATCC 2091 |
250
(1000) {4} |
250
(1000) {4} |
500 (> 1000) {nd} |
– | 0.24* | na | na | na | na |
Candida albicans ATCC 10231 |
250
(1000) {4} |
250
(1000) {4} |
500 (> 1000) {nd} |
– | 0.48* | na | na | na | na |
Candida parapsilosis ATCC 22019 |
500
(1000) {2} |
500
(1000) {2} |
1000 (> 1000) {nd} |
– | 0.24* | na | na | na | na |
Candida glabrata ATCC 90030 | 500 (> 1000) {nd} |
1000 (> 1000) {nd} |
1000 (> 1000) {nd} |
– | 0.24* | na | na | na | na |
Candida krusei ATCC 14243 | 1000 (> 1000) {nd} |
1000 (> 1000) {nd} |
1000 (> 1000) {nd} |
– | 0.24* | na | na | na | na |
The standard compounds used as positive controls: ciprofloxacin (CIP), nitrofurantoin (NIT), cefuroxime (CFX), ampicillin (APC) and pipemidic acid (PA) for bacteria and nystatin (NY*) for fungi. Compounds with bactericidal effect (MBC/MIC ≤ 4) are marked in bold. All the experiments were repeated three times (n = 3) and representative data is presented
nd not determined, na not applicable, “–” no activity