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. 2018 Jun 26;9:673. doi: 10.3389/fphar.2018.00673

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Copyright © 2018 Lu, He, Wang, Gao, Zheng, Zhang, Cao, Wang, Mao and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TSPf induces leukemia cell apoptosis. (A) Leukemia cell lines were treated with 6 μg/ml TSPf or DMSO for 24 h followed by lysate preparation and immunoblotting analysis against PARP and GAPDH. (B,C) K562 and HL-60 cells were treated with TSPf at indicated concentrations (B) for 24 h or 4 μg/ml of TSPf for indicated periods (C) followed by analysis for PARP. GAPDH was used as a loading control. (D) K562 and HL-60 cells were treated with TSPf at indicated concentrations for 24 h followed by immunoblotting assays against caspase 3. GAPDH was used as an internal loading control.