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. 2018 Jun 26;9:673. doi: 10.3389/fphar.2018.00673

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Copyright © 2018 Lu, He, Wang, Gao, Zheng, Zhang, Cao, Wang, Mao and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TSPf suppresses the RNF6/AKT/mTOR pathway in tumor tissues. At the end of the experiment, tumor tissues were dissected and frozen in liquid nitrogen immediately and stored at –80°C for further assays. Total proteins were extracted from the tumor tissues followed by immunoblotting assays against typical pro-survival and pro-apoptotic proteins (A), the AKT and mTOR proteins and their phosphorylation levels (B), the autophagic proteins LC3 and p62 (C). (D) K562 and HL-60 cells were treated with TSPf at indicated concentration for 24 h, followed by immunoblotting for LC3 and p62.