REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-V5 (Mouse)	BioRad	Cat# MCA1360
Anti-HA 3F10 (Rat)	Roche	Cat# 11867423001
Anti-PGK1 22C5D8 (Mouse)	Life Technologies	Cat# 459250
Anti-GFP	Roche	Cat# 1184460001
Bacterial and Virus Strains
BL21(DE3) RIPL	Life technologies	Cat# C6000-03
DH10EmbacY	Vijayachandran et al., 2011	N/A
Chemicals, Peptides, and Recombinant Proteins
Acid-washed glass beads	Sigma	Cat# G8722
Complete EDTA free protease inhibitor cocktail	Roche	Cat# 4693132001
Indole-3-acetic acid (IAA/Auxin)	Sigma	Cat# I3750-5G-A
Immobilon Western ECL	Millipore	Cat# WBLKS0500
Nocodazole	Sigma	Cat# M1404
PMSF	Sigma	Cat# 329-98-6
Proteinase K	Roche	Cat# 03115836001
RNase A	Roche	Cat# 10109169001
α-factor peptide	CRUK Peptide Synthesis Service	N/A
FuGENE HD Transfection reagent	Promega	Cat# E2311
Cre Recombinase	New England Biolabs	Cat# M0298S
NuPAGE 3-8% Tris-Acetate Protein Gels, 1.5 mm, 10-well	ThermoFisher	Cat# EA0378BOX
NuPAGE 4-12% Bis-Tris Protein Gels, 1.0 mm, 10-well	ThermoFisher	Cat# NP0321BOX
Protein G dynabeads	ThermoFisher	Cat# 10003D
Dynabeads M-280 Streptavidin	Invitrogen	Cat# 11205D
DNaseI	Roche	Cat# 04716728001
StrepTrap HP	Fisher Scientific	Cat# 11540654
Desthiobiotin	Fisher Scientific	Cat# 12753064
Superose 6 Increase 10/300 GL	VWR	Cat# 29-0915-96
HiLoad 16/60 Superdex 200	GE Healthcare	Cat# GE28-9893-35
Cellfectin II Reagent	Invitrogen	Cat# 10362100
Antibiotics Antimycotic Solution	Sigma Aldrich	Cat# A5955
Fetal Bovine Serum	Sigma Aldrich	Cat# 12303C
SF-900 III SFM	Life technologies	Cat# 12658027
Ni Sepharose 6 Fast Flow	GE Healthcare	Cat# 17-5318-02
Glutathione Sepharose 4B	GE Healthcare	Cat#17075605
Critical Commercial Assays
AxyPrep Mag PCR Clean-up kit	Appleton Woods Ltd	Cat# AX402
ChIP Clean and Concentrator kit	Zymo Research	Cat# D5205
E-Gel SizeSelect II Agarose Gels, 2%	ThermoFisher	Cat# G661012
EnzChek phosphate assay kit	Invitrogen	Cat# E6646
Library Quantification Kit Ion Torrent Platforms	KAPA Biosystems	Cat# KR0407
NEBNext Fast DNA Library Prep Set for Ion Torrent kit	New England Biolabs	Cat# E6270L
TnT Quick Coupled Transcription/Translation System	Promega	Cat# L2080
Deposited Data
Raw and analyzed data (GEO accession number)	This study	GSE106182
Experimental Models: Cell Lines
Sf9 cells in Sf-900 II SFM	ThermoFisher	Cat# 11496015
Experimental Models: Organisms/Strains
Yeast strains used in this study (S. cerevisiae W303/K699, C. glabrata)	Table S2	N/A
Recombinant DNA
pACEbac1 SMC1	This study	N/A
pACEbac1 8His-SMC3	This study	N/A
pIDC SCC1-2xStrepII	This study	N/A
pIDC SCC3	This study	N/A
pIDS PDS5-Flag	This study	N/A
pIDS WAPL	This study	N/A
pACEbac1 SMC1-8xHis-SMC3	This study	N/A
pIDC SCC1-2xStrepII-SCC3	This study	N/A
pIDS PDS5-Flag-WAPL	This study	N/A
pACEbac1 SMC1-8xHis-SMC3 / pIDC SCC1-2xStrepII (trimer)	This study	N/A
pACEbac1 SMC1-8xHis-SMC3 / pIDC SCC1-2xStrepII-SCC3 (tetramer)	This study	N/A
pACEbac1 SMC1-8xHis-SMC3 / pIDC SCC1-2xStrepII-SCC3 / pIDS PDS5-Flag-WAPL (hexamer)	This study	N/A
pACEbac1 GFP-(Δ1-132)SCC2-StrepII	This study	N/A
pGEX6p-1 GST-Scc2171-1504	This study	N/A
pFastbacHT 6xHis-Pds5	This study	N/A
pCS2 Scc11-566	This study	N/A
pCS2 Scc11-566Δ152-256	This study	N/A
pCS2 Scc11-256	This study	N/A
pACEbac1 SMC1-8xHis-SMC3 / pIDC scc1(V137K)-2xStrepII-SCC3 (tetramer)	This study	N/A
pACEbac1 GFP-(Δ1-132)scc2(K788E H789E)-StrepII	This study	N/A
pACEbac1 GFP-(Δ1-132)scc2(E822K L937F)-StrepII	This study	N/A
pACEbac1 SMC1-8xHis-SMC3 / pIDC scc1(Δ152-256) -2xStrepII-SCC3 (tetramer)	This study	N/A
pACEbac1 SMC1-8xHis-SMC3 / pIDC scc1(Δ152-206, Δ244-251) -2xStrepII-SCC3 (tetramer)	This study	N/A
pACEbac1 smc1(E1158Q)-8xHis-smc3(E1155Q) / pIDC SCC1-2xStrepII-SCC3 / pIDS PDS5-Flag-WAPL (hexamer)	This study	N/A
pACEbac1 SMC1-8xHis-SMC3 / pIDC scc1(Δ196-203, Δ244-251, Δ281-288)-2xStrepII-SCC3 (tetramer)	This study	N/A
pACEbac1 SMC1-8xHis-SMC3 / pIDC scc1(Δ196-203, Δ244-251)-2xStrepII-SCC3 (tetramer)	This study	N/A
Software and Algorithms
Galaxy platform	Giardine et al., 2005	https://usegalaxy.org
FastQC	Galaxy tool version 1.0.0	https://usegalaxy.org
Trim sequences	Galaxy tool version 1.0.0	https://usegalaxy.org
Filter FASTQ	Galaxy tool version 1.0.0	https://usegalaxy.org
Bowtie2	Langmead and Salzberg, 2012; Galaxy tool version 0.2	https://usegalaxy.org
Bam to BigWig	Galaxy tool version 0.1.0	https://usegalaxy.org
Samtools	Li et al., 2009	http://samtools.sourceforge.net/
IGB browser	Nicol et al., 2009	http://bioviz.org/igb/
Filter SAM or BAM	Li et al., 2009; Galaxy tool version 1.1.0	https://usegalaxy.org
chr_position.py	This paper	https://github.com/naomipetela/nasmythlab-ngs
filter.py	This paper	https://github.com/naomipetela/nasmythlab-ngs
bcftools call	Li et al., 2009	N/A
MutationFinder.py	This paper	N/A
yeastmine.py	This paper	N/A