Figure 6.

DAAM1 and RHOA participate in the rearrangement of stress fibers. A, DAAM1 shRNA, CCG-1423 treatment, or cyclo(-RGDfK) treatment disrupted the formation of actin stress fibers in MDA-MB-231 cells. Stable DAAM1 knockdown MDA-MB-231 cells were grown on collagen-coated coverslips. MDA-MB-231 cells grown on collagen-coated coverslips were pretreated with 1 μmol/liter CCG-1423 or 20 nmol/liter cyclo(-RGDfK) for 1 h. Subsequently, cells were fixed, and F-actin organization was analyzed by phalloidin staining. Bar, 10 μm. Objective lens, magnification, ×40; numerical aperture, 0.95. B and C, DAAM1 shRNA, CCG-1423, or cyclo(-RGDfK) disrupted the formation of stress fibers in MDA-MB-231 cells. The number of stress fibers in a cell (B) and the length of the longest microfilament (C) were determined in MDA-MB-231 cells (n = 20). Error bars, S.D.