Table 1.
Sequences of the primers used in the study
| Primer | Sequence |
|---|---|
| Rv3137_Fpa | 5′-CACCCATATGCACCATCATCATCATCATGTGAGCCACGACGATCTAATG-3′ |
| Rv3137_Rpa | 5′-TATAAGCTTTTACCCGGCGTTGAGCCGTGT-3′ |
| Asp44-Ala_Fp | 5′-CGACCGCCCGAGCGGCGTCGGTC-3′ |
| Asp44-Ala_Rp | 5′-GACCGACGCCGCTCGGGCGGTCG-3′ |
| Glu67-Ala_Fp | 5′-CCGCCGAACTCCGCGCCCAAGACGC-3′ |
| Glu67-Ala_Rp | 5′-GCGTCTTGGGCGCGGAGTTCGGCGG-3′ |
| Asp83-Ala_Fp | 5′-CAGTGGATCGTAGCCCCGATCGACGGC-3′ |
| Asp83-Ala_Rp | 5′-GCCGTCGATCGGGGCTACGATCCACTG-3′ |
| Thr88-Ala_Fp | 5′-GCACAAAGTTTTTGGCGCCGTCGATCGGGTC-3′ |
| Thr88-Ala_Rp | 5′-GACCCGATCGACGGCGCCAAAAACTTTGTGC-3′ |
| Asp213-Ala_Fp | 5′-CAGTGCCGCCAGAGCCCATACCGACAC-3′ |
| Asp213-Ala_Rp | 5′-GTGTCGGTATGGGCTCTGGCGGCACTG-3′ |
a Underlined sequences represent restriction sites. Italic sequences represent His6 tag sequence. Sequences in bold are Rv3137-specific N- and C-terminal sequences. Fp and Rp stand for forward and reverse primer, respectively.