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. 2018 May 16;293(26):10128–10140. doi: 10.1074/jbc.RA118.002399

Figure 4.

Figure 4.

Loss of Ei24 disrupts calcium homeostasis. A, mutation in Ei24 generated by CRISPR/Cas9 in INS1 cells. B, Western blotting results demonstrating that Ei24 is effectively depleted in Ei24 KO cells. C, intracellular calcium levels were evaluated in single cells at basal conditions by measuring the 340/380 nm ratio of Fura 2-AM (number of cells analyzed: WT, n = 312; KO, n = 282). D, quantification of ER-GCaMP6 fluorescence peak values in responding WT and Ei24 KO cells (number of cells analyzed: WT, n = 38; KO, n = 36 cells). E, left panel, average curve of Fura 2 in WT and KO INS1 cells stimulated with 2 μm TG (number of cells analyzed: WT, n = 57; KO, n = 48 cells). Right panel, area under the curve (A.U.C.) of calcium entry after depletion of the ER with TG treatment. Experiments were independently repeated three times. Data are represented as the mean ± S.D. (error bars). ***, p < 0.001; **, p < 0.05.