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. 2018 May 15;293(26):10275–10286. doi: 10.1074/jbc.RA118.003113

Figure 1.

Figure 1.

CIF3 co-localizes with CIF1 and CIF2 at the new FAZ tip. A, subcellular localization of CIF3 during the cell cycle. Cells were co-immunostained with FITC-conjugated anti-HA mAb and anti-CC2D pAb to detect CIF3–3HA and the FAZ, respectively. The white arrowheads indicate the weak CIF3 fluorescence signal at the old FAZ tip. N, nucleus; K, kinetoplast. Scale bar: 5 μm. B, CIF3 co-localizes with CIF1 at the new FAZ tip from S phase to early cytokinesis. Cells expressing endogenously 3HA-tagged CIF3 and PTP-tagged CIF1 were co-immunostained with FITC-conjugated anti-HA mAb and anti-protein A pAb. The white arrowheads indicate the weak CIF3 fluorescence signal at the old FAZ tip. Scale bar: 5 μm. C, CIF3 co-localizes with CIF2 at the new FAZ tip during S phase. Cells co-expressing CIF3–3HA and CIF2–PTP from their respective endogenous locus were co-immunostained with FITC-conjugated anti-HA mAb and anti-protein A pAb. Scale bar: 5 μm.