Figure 8.

Summary of the phenotypes of CIF1, CIF2, and CIF3 RNAi and a mode of action of cytokinesis regulators at the new FAZ tip in T. brucei. A, summary of the phenotypes caused by CIF1 RNAi, CIF2 RNAi, and CIF3 RNAi and the effect on the stability of the three proteins in the RNAi cell lines. B, a mode of action of cytokinesis regulators at the new FAZ tip and the cleavage furrow. Purple arrows indicate the duration of the time of the cytokinesis regulators at the new FAZ tip during the cell cycle, whereas blue arrows indicate the duration of the cytokinesis regulators at the cleavage furrow. Cell cycle stages are indicated at the left. Black arrows indicate the positive effect by TbPLK, the CIF1–CIF2 complex, the CIF1–CIF3 complex, and TbAUK1, whereas the red arrow indicates the inhibitory effect by CIF1.