Figure 10.

Proposed distribution of GlcUA and IdoUA in CS/DS from HCC70 cells and CCD-1095Sk cells primed on XylNap. A and B, proposed structures of XylNap-primed CS/DS from HCC70 cells (A) and CCD-1095Sk cells (B). The distribution of IdoUA and GlcUA was based on the MS data of the main products generated after chondroitinase AC-I and -II and chondroitinase B degradation. Two parallel lines indicate that the chain either ends or continues with the following polysaccharide. B, different co-polymeric CS/DS variants illustrate possible GlcUA and IdoUA distributions as repeating patterns rather than established structures. The length of the GAGs was based on previous results (34), corresponding to an average length of 30 disaccharides. Sulfation was not taken into consideration.