Figure 5.

Linkage region products generated after chondroitinase ABC degradation of XylNap-primed CS/DS from HCC70 cells and CCD-1095Sk cells. A–F, average HCD-MS² spectra of the precursor ions at m/z 607.64 [2−] corresponding to a naphthyl-containing hexasaccharide linkage region modified with one sulfate group (A and B; L6S1 in Table 1), at m/z 647.62 [2−] corresponding to one with two sulfate groups (C and D; L6S2 in Table 1), and at m/z 753.19 [2−] corresponding to one carrying one sulfate group and one Neu5Ac residue (E and F; L6S1SA1 in Table 1) from chondroitinase ABC-degraded XylNap-primed GAGs from HCC70 cells (A, C, and E) and CCD-1095Sk cells (B, D, and F). G and H, relative abundance ratios of precursor ions of the observed linkage region variants primed on XylNap (light) and XylNap-d₇ (heavy) generated after chondroitinase ABC degradation of 1:1 mixed samples from HCC70 cells (red) and CCD-1095Sk cells (blue). G, HCC70 sample was heavy; H, CCD-1095Sk sample was heavy.