Figure 9.

NREs common to XylNap-primed GAGs from both HCC70 cells and CCD-1095Sk cells. A–C, average HCD-MS² spectra of the NRE precursor ions at m/z 380.00 [1−] (A), 476.07 [1−] (B), and 490.09 [1−] (C) observed in chondroitinase ABC-degraded XylNap-primed GAGs from both HCC70 cells and CCD-1095Sk cells. D, incorporation of [³H]methyl in XylNap-primed GAGs from HCC70 cells and CCD-1095Sk cells expressed as radioactivity in dpm per μg of GAGs. E, average HCD-MS² spectrum of the NRE precursor ion at m/z 556.01 [1−] observed in chondroitinase AC-I– and -II–degraded XylNap-primed GAGs from HCC70 cells. The nomenclature of the fragment ion at m/z 198.99 was based on that described by Domon and Costello (60).