Table 1.
Summary of oligosaccharides and linkage regions of XylNap-primed GAGs from HCC70 cells and CCD-1095Sk cells identified by LC-MS/MS after degradation with chondroitinase AC-I and -II (AC), or chondroitinase B (B)
| Cell line | Degradation enzyme | Precursor ion mass | Negative charge | Glycan compositiona | |
|---|---|---|---|---|---|
| HCC70 | CCD-1095Sk | AC; B | 458.06 | 2 | dp4S2 |
| HCC70 | CCD-1095Sk | AC | 498.04 | 2 | dp4S3 |
| HCC70 | CCD-1095Sk | AC | 562.61 | 2 | dp4S3b |
| CCD-1095Sk | B | 562.61 | 2 | dp4S3b | |
| HCC70 | CCD-1095Sk | AC; B | 458.06 | 3 | dp6S3 |
| HCC70 | CCD-1095Sk | AC; B | 752.17 | 2 | dp6S3b |
| HCC70 | CCD-1095Sk | AC | 757.22 | 1 | L4 |
| HCC70 | CCD-1095Sk | AC; B | 837.17 | 1 | L4S1 |
| HCC70 | CCD-1095Sk | AC | 523.65 | 2 | L4SA1 |
| HCC70 | CCD-1095Sk | AC | 563.63 | 2 | L4S1SA1 |
| HCC70 | B | 563.63 | 2 | L4S1SA1 | |
| HCC70 | CCD-1095Sk | AC | 567.66 | 2 | L6 |
| HCC70 | CCD-1095Sk | AC; B | 607.64 | 2 | L6S1 |
| HCC70 | CCD-1095Sk | AC; B | 647.61 | 2 | L6S2 |
| CCD-1095Sk | B | 687.59 | 2 | L6S3 | |
| HCC70 | CCD-1095Sk | AC; B | 753.19 | 2 | L6S1SA1 |
| HCC70 | AC | 793.16 | 2 | L6S2SA1 | |
| HCC70 | CCD-1095Sk | AC; B | 797.19 | 2 | L8S1 |
| HCC70 | CCD-1095Sk | B | 837.17 | 2 | L8S2 |
| HCC70 | AC | 837.17 | 2 | L8S2 | |
| CCD-1095Sk | B | 557.78 | 3 | L8S2 | |
| CCD-1095Sk | AC; B | 941.73 | 2 | L8S3* | |
| HCC70 | CCD-1095Sk | B | 982.72 | 2 | L8S2SA1 |
| HCC70 | AC | 982.72 | 2 | L8S2SA1 | |
| CCD-1095Sk | B | 986.75 | 2 | L10S1 | |
| HCC70 | CCD-1095Sk | B | 1026.73 | 2 | L10S2 |
| CCD-1095Sk | B | 684.15 | 3 | L10S2 | |
| CCD-1095Sk | AC | 710.80 | 3 | L10S3 | |
| HCC70 | B | 710.80 | 3 | L10S3 | |
| HCC70 | CCD-1095Sk | AC; B | 1131.28 | 2 | L10S3b |
| CCD-1095Sk | B | 1235.84 | 2 | L10S4b | |
| HCC70 | B | 807.83 | 3 | L10S3SA1 | |
| CCD-1095Sk | B | 1216.28 | 2 | L12S2 | |
| CCD-1095Sk | B | 810.52 | 3 | L12S2 | |
| HCC70 | CCD-1095Sk | B | 837.17 | 3 | L12S3 |
| HCC70 | CCD-1095Sk | B | 1320.84 | 2 | L12S3b |
| CCD-1095Sk | B | 647.62 | 4 | L12S4 | |
| CCD-1095Sk | B | 1425.39 | 2 | L12S4b | |
| HCC70 | B | 906.87 | 3 | L12S4b | |
| HCC70 | B | 1003.90 | 3 | L12S4SA1b | |
| CCD-1095Sk | B | 963.54 | 3 | L14S3 | |
| HCC70 | CCD-1095Sk | B | 1033.24 | 3 | L14S4b |
| HCC70 | CCD-1095Sk | B | 1102.95 | 3 | L14S5b |
| CCD-1095Sk | B | 1063.26 | 3 | L16S2 | |
| CCD-1095Sk | B | 1159.61 | 3 | L16S4b | |
| CCD-1095Sk | B | 1229.32 | 3 | L16S5b | |
| HCC70 | CCD-1095Sk | B | 1299.02 | 3 | L16S6b |
a Glycans were identified using the algorithm described under “Experimental procedures.” Glycans were ordered according to their glycan compositions and characterized by their precursor ion masses and net charges. Dp is degree of polymerization; L is linkage region; n is number of repeats; S is sulfate; SA is Neu5Ac.
b These are dibutylamine adducts.