Figure 2.

Splice isoforms of Ano1 and Ano2 in the VNO. A and B, RT-PCR on VNO tissue probing different Ano1 (A) or Ano2 (B) isoforms. The white numbers indicate target exons of forward (fwd) and reverse (rev) primers hybridizing to the spliced-together exons. Schematic drawings of the respective transcript variants are shown below, exon-spanning forward primers are indicated as red arrows, and variants that were not detected in the VNO are faded. Transcripts of the ubiquitously expressed enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were amplified as positive control.