Figure 5.

Activity of N. benthamiana A1.1 and Fabrazyme toward 4MU-α-GAL and human glycosphingolipids. A, 4MU–α-Gal activities of pure A1.1 and Fabrazyme at different pH values (3.8–8). B, Michaelis-Menten kinetic values of both enzymes toward 4MU–α-Gal, NBD-Gb3, and lyso-Gb3 substrates. Data represent mean values, n ≥ 2. C, degradation of 5 μm NBD-Gb3 to NBD-LacCer by 50 ng of A1.1 or Fabrazyme at varying pH values (4.5–7.5). Quantification of HPTLC plates were conducted more than once, showing one gel quantification.