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. 2018 May 10;293(26):10353–10362. doi: 10.1074/jbc.RA118.002889

Figure 5.

Figure 5.

A dearth of activating chromatin marks at orphan IRF1 sites in HeLa cells. ChIP–qPCR was performed to detect STAT1, IRF1, and the indicated coactivators or epigenetic marks in HeLa cells 0, 6, and 24 h after IFNγ treatment. Tested sites included 7 positive controls (ctrls), 4 negative controls at the CIITA and SOCS1 loci, and 12 randomly selected orphan IRF1 sites (Or1–Or12). *, site at which the IFNγ-induced signal is >2-fold above basal levels at the same site and also >2-fold above the signal at the negative control +46 kb site near SOCS1 after treatment. #, site at which basal levels are >2-fold above basal levels at the +46 kb negative control site. The data are expressed as percentages of input and are from at least two independent experiments, and error bars indicate the range.