FIGURE 10.

Commd10 knockdown decreases Tf recycling. Control and C10 knockdown cells were incubated with 50 μg/mL Tf-546, or without Tf-546 (A), in full growth media for 5 min (B) before media was removed and cells were incubated with PBS at 37°C for 15 (C) or 30 (D) minutes to “chase” the transferrin through the recycling pathway. Scale bar = 15 μm. Repeated measure two-way ANOVA with Sidak’s post hoc test. ^∗Significance between individual scoring levels of control and Commd10 knockdown cells (i.e., level one between control and Commd10 knockdown cells at one timepoint). #Significance between total spread of control and Commd10 knockdown cells at one timepoint. n = 3, total number of cells analyzed n = 108–237. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, ^∗∗∗∗P < 0.0001. ^###P < 0.001, ^####P < 0.0001.