FIGURE 5.

Rescue of Commd10 knockdown. Control and Commd10 knockdown FRT epithelia were transfected with plasmids encoding the α-, β_HA-, and γ-ENaC subunits and a plasmid encoding COMMD10 (A,B). FRT control and Commd10 KD cells were lysed and Commd1 protein levels were determined by immunoblotting and quantified using densitometry (C). Data shown as mean ± SD relative to β-actin loading control and FRT control. One-sample t-test, ^∗P < 0.05, n = 4. Control and Commd10 knockdown FRT epithelia were transfected with plasmids encoding the α-, β_HA-, and γ-ENaC subunits and a plasmid encoding COMMD1 (D,E). I_sc-amil was measured and quantified, one-way ANOVA with Tukey’s post hoc test, ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗∗P < 0.0001, n = 4–7.