FIGURE 6.

Brefeldin A has a cumulative effect on I_sc-amil in C10 KD epithelia. (A) Experimental design. (B) Representative I_sc profile for control and Commd10 KD epithelia transfected with plasmids encoding the α-, β_HA-, and γ-ENaC subunits with addition of amiloride prior to incubation with BFA. (C) Representative I_sc profile for control and Commd10 KD epithelia after 20 min incubation with BFA or vehicle. (D) Pooled I_sc-amil for control and Commd10 KD epithelia prior to, and after, the addition of BFA or vehicle. Data shown as mean ± SD relative to I_sc-amil in FRT control epithelia prior to BFA incubation. One-way ANOVA with multiple comparisons and Tukey’s post hoc test. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, n = 4.