Fig. 5.

P3HB4HB production by E. coli strains grown in shake flasks. E. coli recombinants harboring different plasmids were cultivated in MM medium supplemented with 10 g/L yeast extract and different carbon sources at 37 °C for 48 h. Initial acetate (A) concentration was 5 g/L. α-ketoglutarate (KG) or citrate (C) was added as assistant carbon source at a concentration of 1 g/L. CDW (a), P3HB4HB content (b), P3HB4HB titer (c), and rCDW (d) were measured. The columns represent the averages of triplicate experiments, and the error bars represent standard deviation