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. 2018 Jul 3;49:54. doi: 10.1186/s13567-018-0547-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Bovine enteroids cultivated in Intesticult medium alone. A Morphology of freshly isolated bovine small intestinal crypts. B Representative images showing the growth and development of an individual enteroid cultivated in Intesticult medium alone during 11 days of culture. Arrows, crypt buds. C Enteroids cultivated in Intesticult medium alone did not survive after subsequent passage (P1: passage 1; d1: day 1 of culture).