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. 2018 Jul 3;49:54. doi: 10.1186/s13567-018-0547-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Histological analysis of bovine enteroids. A Representative Z axis-projections of enteroids whole-mount stained to detect cell nuclei (DAPI, blue). B Detection of F-actin-expressing brush borders at the enteroid lumenal surface (L). C IHC analysis of villin expression in bovine enteroids. D–F, Ulstrastructural analyses of bovine enteroids. E Ultrastructural analyses shows the presence of microvilli (MV) on the apical surface of the enterocytes. The enterocytes are sealed by tight junctions (open arrows) and connected by desmosomes (closed arrows). F Detection of occasional cells containing dense cytoplasmic vesicles indicative of anti-microbial factor secreting Paneth cells. G IHC detect of abundant Ki67⁺ proliferating cells (green).