Fig. 8.

Treatment with the antiangiogenic mβCTPs and knockdown of endogenous β3-endonexin in HUVECs both suppress cell proliferation and VEGFA expression. HUVECs were treated with the indicated mβCTPs (20 μM at a final concentration) or transfected with siRNA (siControl or siβ3-endo). a, b HUVEC proliferation was evaluated using CCK-8 cell proliferation assay. c, d The expression levels of VEGFA mRNA in HUVECs were quantified by qRT-PCR. e, f HUVECs were treated with either siRNAs or mβCTPs as indicated and seeded on polymerized Matrigel in 24-well plates in the absence or presence of VEGF (20 ng/ml). The formed capillary-like structures were counted under an inverted microscope (5× objective). Results were expressed as means ±SD of three experiments; statistical significance was analyzed using Student’s t test (*, p < 0.05; **, p < 0.01)