Fig. 2.
Deletions of known short linear motifs impairs HOG pathway-specific reporter activation. a. Histograms of flow cytometry data showing expression of GFP driven by a Hog1-specific promoter (Stl1) before (lower panel) and after (upper panel) a 60 min induction with 0.4 M NaCl. Pbs2: both a 10 residue deletion (Δ91–100) and double point mutant (P96A + P99A) of SH3 binding domain essentially eliminates reporter output; Hot1: (glycerol biosynthesis transcription factor) 13 residue deletion of a known activation domain (373–385) substantially impairs pathway output - and effects a measurable bimodal distribution; Opy2: 13 residue deletion of known Ste50 binding domain (233–245) effects a partial reduction in reporter output. b. Comparison of distributions using the KL divergence, DKL(P ∣ |Q). The top panel shows cartoon distributions representing either mutant (P) or wild-type (Q) flow cytometry data. The bottom panel shows the log ratio of the two distributions weighted by the probability under the mutant. The KL divergence (equation below the bottom panel) is the integral of this curve (grey area). Dashed line shows that when P(x) and Q(x) have the same value, there is no contribution to KL divergence