Fig. 1.

Fascin regulates melanoma tumorigenesis and stemness. a Western blot analysis of fascin in WM793 cells after treatment with fascin sgRNA and control sgRNA. b WM793 control and fascin KO cells were subjected to soft-agar colony-formation assay, and the colonies were stained with iodonitrotetrazolium chloride. c Quantification of colony numbers per well was shown in bar graph. Data represented as mean ± SD; n = 3 (*P < 0.05). d WM793 control and WM793 fascin KO cells were subjected to sphere formation assay. e Quantification of sphere numbers in WM793 control and WM793 fascin KO cells. Data represented as mean ± SD; n = 3 (***P < 0.001). f Western blot analysis of CD44 expression in control and fascin KO WM793 cells