Fig. 4.

Fascin regulates melanoma sphere formation through TAZ. a WM793 cells were infected with control or TAZ shRNAs. Expression of TAZ was analyzed by Western blot. b Quantification of sphere numbers in control and TAZ knockdown WM793 cells. Data represented as mean ± SD; n = 3 (***P < 0.001). c WM793 fascin KO cells were infected with TAZ-WT and TAZ-S89A. Expression of TAZ was analyzed by Western blot. d Expression of TAZ-WT and TAZ-S89A overrides fascin knockdown effect on sphere growth. Data represented as mean ± SD; n = 3 (***P < 0.001). e WM39 cells were infected by TAZ shRNA and/or fascin-WT. Expression of TAZ and fascin were analyzed by Western blot. f Quantification of sphere numbers in control, TAZ knockdown, fascin overexpression and fascin overexpression; TAZ knockdown WM39 cells. Data represented as mean ± SD; n = 3 (***P < 0.001)